Sequoia Scientific


Harmful algae bloom (HAB) instrument

The LISST-HAB is a self-contained, stand-alone instrument system for use on profiling packages, towed and remote vehicle applications, for deployment during a HAB event. The system will continuously measure particle size distribution and concentration, along with the fluorescence of Phycocyanin (Freshwater HAB’s), Phycoerythrin (Marine HAB’s), Chlorophyll and beam attenuation.


  • Complete package based on Small Angle Forward Scattering Laser Diffraction Technology
  • Measures Particle Size and Concentration in 36 Size Classes
  • Integrated with Turner Designs Cyclops-7F fluorometers engineered to detect Phycocyanin (freshwater HAB’s), Phycoerythrin (marine HAB’s), and Chlorophyll
  • Internal programmable datalogger
  • Small and Extended Battery Packs available for self-contained operation
  • USB connection to PC for programming, offloading and real-time size distribution displays
  • Programmable, autonomous data collection
  • Integrated Depth and Fast Response Temperature Sensors
  • Towable or integrated on profiling package or vehicle

Fluorometer Performance
The Turner Designs submersible instrumentation modules used in the LISST-HAB includes single-channel fluorometers for detection of chlorophyll, freshwater and marine HAB’s. Together with particle information from the LISST, this package solution provides a comprehensive picture of a HAB and its development.


Parameters Measured

  • Particle Size Distribution from 1.0-500 μm in 36 size ranges
  • Depth (600 m max depth @ 0.01 m resolution)
  • Temperature @ 0.01°C resolution; Response time: 2.5 s.
  • Optical transmission @ 0.1% resolution
  • Volume Concentration @ 0.1μl/l resolution
  • Beam transmission from 0.3-0.99 (30-99%)
  • Phycocyanin (freshwater HAB’s)
  • Phycoerythrin (marine HAB’s)
  • Chlorophyll

Operating Concentration range

  • Optical transmission from 0.3-0.99 (30-99%)
  • Concentration from ~ 0.5-700 mg/l (particle-size dependent)
Fluorometer ranges
Chlorophyll 0.03 ug/l 0-500 ug/l

Technology (laser diffraction)

  • Small-angle forward scattering
  • 32-ring custom photodiode Ring Detector + 4 large angle detectors
  • 25 mm optical path

Mechanical and Electrical

  • Dimensions: 63.9 cm (25.2”) L x 10.03 cm (3.95”) Ø
  • Weight: 6 kg (13.2 lbs) in air; 2.5 kg (5.5 lbs) in water
  • 600 m depth rating
  • External power input: 12VDC nominal, 8-24 VDC
  • Sampling rate: Up to 1 Hz
  • Current drain at 12 V: 100 mA sampling, 8 mA between samples.
  • Data storage: 1GB (~12,500,000 measurements)
  • Connectors: S/S Impulse MCBH(WB)-3-MP, MCBH(WB)-5-MP, MCBH(WB)-6-MP
  • Laser: 670 nm solid state diode laser
  • Chlorophyll – EX 465 nm, EM 496 nm
  • Phycocyanin – EX , EM
  • Phycoerythrin – EX , EM


What is the LISST-HAB exactly?

The LISST-HAB is a LISST-200X integrated with three Turner Designs fluorometers, sensitive to algae pigments and Chlorophyll. The fluorometers are powered by the LISST-200X, which also controls their sampling. The fluorometer signals are then read by and integrated into the LISST-200X datastream for a unified dataset.