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Sequoia Scientific

LISST-HAB

Harmful algae bloom (HAB) instrument

The LISST-HAB is a self-contained, stand-alone instrument system for use on profiling packages, towed and remote vehicle applications, for deployment during a HAB event. The system will continuously measure particle size distribution and concentration, along with the fluorescence of Phycocyanin (Freshwater HAB’s), Phycoerythrin (Marine HAB’s), Chlorophyll and beam attenuation.

Features

  • Complete package based on Small Angle Forward Scattering Laser Diffraction Technology
  • Measures Particle Size and Concentration in 36 Size Classes
  • Integrated with Turner Designs Cyclops-7F fluorometers engineered to detect Phycocyanin (freshwater HAB’s), Phycoerythrin (marine HAB’s), and Chlorophyll
  • Internal programmable datalogger
  • Small and Extended Battery Packs available for self-contained operation
  • USB connection to PC for programming, offloading and real-time size distribution displays
  • Programmable, autonomous data collection
  • Integrated Depth and Fast Response Temperature Sensors
  • Towable or integrated on profiling package or vehicle

Fluorometer Performance
The Turner Designs submersible instrumentation modules used in the LISST-HAB includes single-channel fluorometers for detection of chlorophyll, freshwater and marine HAB’s. Together with particle information from the LISST, this package solution provides a comprehensive picture of a HAB and its development.

Specifications

Parameters Measured

  • Particle Size Distribution from 1.0-500 μm in 36 size ranges
  • Depth (600 m max depth @ 0.01 m resolution)
  • Temperature @ 0.01°C resolution; Response time: 2.5 s.
  • Optical transmission @ 0.1% resolution
  • Volume Concentration @ 0.1μl/l resolution
  • Beam transmission from 0.3-0.99 (30-99%)
  • Phycocyanin (freshwater HAB’s)
  • Phycoerythrin (marine HAB’s)
  • Chlorophyll

Operating Concentration range

  • Optical transmission from 0.3-0.99 (30-99%)
  • Concentration from ~ 0.5-700 mg/l (particle-size dependent)
Fluorometer ranges
Minimum
Detection
Linear
Range
Phycocyanin
Phycoerythrin
Chlorophyll 0.03 ug/l 0-500 ug/l

Technology (laser diffraction)

  • Small-angle forward scattering
  • 32-ring custom photodiode Ring Detector + 4 large angle detectors
  • 25 mm optical path

Mechanical and Electrical

  • Dimensions: 63.9 cm (25.2”) L x 10.03 cm (3.95”) Ø
  • Weight: 6 kg (13.2 lbs) in air; 2.5 kg (5.5 lbs) in water
  • 600 m depth rating
  • External power input: 12VDC nominal, 8-24 VDC
  • Sampling rate: Up to 1 Hz
  • Current drain at 12 V: 100 mA sampling, 8 mA between samples.
  • Data storage: 1GB (~12,500,000 measurements)
  • Connectors: S/S Impulse MCBH(WB)-3-MP, MCBH(WB)-5-MP, MCBH(WB)-6-MP
  • Laser: 670 nm solid state diode laser
  • Chlorophyll – EX 465 nm, EM 496 nm
  • Phycocyanin – EX , EM
  • Phycoerythrin – EX , EM

Accessories

LISST-HAB Item # and Description

Item #DescriptionThumbnail
SEQ-FP-HABLISST-HAB (Harmful Algae Bloom) Instrument
LISST-200X based sensor package with integrated HAB fluorometers. Self-contained, stand-alone system for use on profiling packages, towed and remote vehicle applications, for deployment during a HAB event. Measures particle size distribution, concentration, along with the fluorescence of Phycocyanin (Freshwater HAB’s), Phycoerythrin (Marine HAB’s), Chlorophyll.
LISST-HAB - covered

LISST-HAB - covered

LISST-HAB Accessories *NOT INCLUDED* with LISST-HAB

Item #DescriptionThumbnail
SEQ-AC-L200X-PRMOptical Path Reduction Module for LISST-200X
Extends concentration range by factor of five by reducing the optical path from 25mm to 5mm. Included fused quartz cylinder and mounting hardware.
NOTE: The standard Background Test Chamber for LISST-200X will not work with the PRM in place. It is highly recommended to use the Large Volume Test Chamber for obtaining a background with the PRM installed.
SEQ-AC-L200X-CHLGLarge Volume Test Chamber for LISST-200X
Clear acrylic chamber with O-ring seal and drain. Used for obtaining clean water backgrounds with Path Reduction module installed.
SEQ-AC-L200X-SCOOPScoop for LISST-200X optics
For applications in rivers and other regions where the LISST-200X is pointed into the flow. Ensures a diverted flow of water through the optical path of the LISST-200X. Includes s/s bolts and nuts.
SEQ-AC-L200X-FRMMounting Frame for LISST-200X & Small Battery
316 s/s frame. Includes:
Frame
Bridle
Small battery housing clamps (set of two clamps)
SEQ-AC-L200X-FRM-CLPSmall battery housing clamp (set of two clamps)
NOTE: Clamps are included with the LISST-200X frame.
SEQ-AC-L200X-SCBL-MMLISST-200X to SBE-9 CTD cable
2 m cable with Impulse 6-pin MCIL-6-FS connectors on each end, wired pin to pin
Allows connection of LISST-200X to Sea Bird CTD with Impulse MCBH connectors.
SEQ-AC-L200X-SCBLB-AMLISST-200X to SBE-9 CTD cable
2 m cable with Impulse 6-pin MCIL-6-FS connector on one end, and Impulse AG206-FS connector on opposite end, wired pin to pin.
Allows connection of LISST-200X to Sea Bird CTD with Impulse AG connectors.
SEQ-AC-L200X-CHFPFull Path Flow Through Chamber for LISST-200X
Flow through chamber that allow pumped water to pass through the optics of the LISST-200X. Simple to install. Includes barb fittings for 1/2” ID tubing. Compatible with standard 1/2” NPT fittings.
SEQ-AC-L200X-CBLBConnector Base – Custom length integrated Communication and external power cable for LISST-200X/-100X/-STX/-Deep
Includes built-in USB adapter that allows the instruments to be powered from PC USB port.
Note: must specify length of cable required by ordering item below
SEQ-AC-L200X-CBLCustom length integrated Communication and external power cable for LISST-200X/-100X/-STX/-Deep
Customer to specify cable length. Must order with SEQ-AC-L200X-CBLB. E.g. to order a 35 m cable:
1 x SEQ-AC-L200X-CBLB
35 x SEQ-AC-L200X-CBL
SEQ-AC-L200X-DPSRTowed Underwater Depressor
Designed to secure the LISST-200X instrument into a tow-body configuration, allowing the ability to be deployed from all types of watercraft working at speed. Includes tow-body and installation brackets for securing. Weight in air 25 lbs.

LISST-HAB Accessories *INCLUDED* w/ LISST-HAB

Item #DescriptionThumbnail
SEQ-AC-L200X-LEBLarge External Battery Housing for LISST-200X
Anodized aluminum housing for external battery pack with underwater connectors for mating with LISST-200X. Includes 16 non-rechargeable alkaline D cells installed. Suitable for longer deployments. 600 m depth rating.
*Included as part of LISST-200X standard package.* Does not include cable SEQ-AC-L200X-BCS to connect to LISST-200X.
NOTE: Ships in a corrugated cardboard box.
The price does *NOT* include shipping container (e.g. SEQ-AC-L200X-SC).
SEQ-AC-L200X-SEBSmall External Battery Housing for LISST-200X
Plastic housing for external battery pack containing 2 rechargeable NiMH D cells with underwater connectors for mating with LISST-200X. Includes 4 rechargeable NiMH D cells and Battery Charger. 600 m depth rating.
*Included as part of LISST-200X standard package.* Does not include cable SEQ-AC-L200X-BCS to connect to LISST-200X
NOTE: Ships in a corrugated cardboard box.
The price does *NOT* include shipping container (e.g. SEQ-AC-L200X-SC).
SEQ-AC-L200X-SBATLISST-200X Rechargeable Batteries for Small Battery Pack
NiMH D cells, Qty. 4
*Included as part of LISST-200X standard package*
SEQ-AC-L200X-SCHGRLISST-200X Battery Charger for NiMH D Batteries
*Included as part of LISST-200X standard package*
SEQ-AC-L200X-BCSShort Cable for connecting Small or Large LISST-200X battery housing to LISST-200X
0.5 m long, 5-pin Male to 5-pin Female
*Included as part of LISST-200X standard package*
SEQ-AC-L200X-CHZSBackground Test Chamber for LISST-200X
Test chamber which holds small volume of water around optics while clean water background is obtained.
*Included as part of LISST-200X standard package*
SEQ-AC-L200X-CBL02Integrated Communication and external power cables for LISST-200X/-100X/-STX/-Deep
2 m length. Includes black-jacketed cable spliced to the single communications/power connector. Includes built-in USB adapter that allows the instruments to be powered from PC USB port.
*Included as part of LISST-200X standard package.*
SEQ-AC-L200X-SCShipping Case for LISST-200X
Plastic shipping case with custom cut foam for LISST-200X. Also has cut outs for Small and Large Batteries, spare batteries, Battery Charger, and short cables.
*Included as part of LISST-200X standard package.*

FAQ

What is the LISST-HAB exactly?

The LISST-HAB is a LISST-200X integrated with three Turner Designs fluorometers, sensitive to algae pigments and Chlorophyll. The fluorometers are powered by the LISST-200X, which also controls their sampling. The fluorometer signals are then read by and integrated into the LISST-200X datastream for a unified dataset.

Do the fluorometers report CONCENTRATIONS of the different fluorescent pigments?

No. The data you get from the LISST-HAB fluorometers are raw voltages. You must convert those to pigment concentrations yourself by calibrating the fluorometers with pigment samples.

Fluorometers do not provide perfect measurements of concentration: They are imperfect proxies for the specific compounds. Calibrating them involves your scientific judgement, and that may be different for different environments. For this reason Sequoia does not calibrate them in advance.